1364 Potential role of protein coactosin-like protein (COTL-1) and fibrinogen (FGB) in hidradenitis suppurativa possibly through enhancing vessel permeability and promoting keratinocyte growth

Backgrounds: Hidradenitis suppurativa (HS) is a chronic, autoinflammatory, and devastating disease that preferentially affects the intertriginous sites. The profile of blood inflammatory protein known to associate with development clinical severity autoinflammatory diseases. However, current understanding proteins their pathogenic roles in HS remains limited. Objective: purpose this study was candidate serum potential mechanism HS. Methods: We conducted iTRAQ gel-free proteomics screen from patients healthy controls followed by validation ELISA. were tested leukocyte trans-endothelial model, vascular permeability test keratinocyte proliferation (WST-1 assay) investigate mechanistic these proteins. Results: showed human fibrinogen beta (FGB) which significantly elevated patients. Serum enhanced proliferation, also induced FGB treatment at 96 hours. In parallel, we increased vitro. Coactosin-like protein(COTL1) time-course dependent dosage manner. Conclusion: Human COTL-1 may contribute pathophysiology increasing enhance proliferation.